Mouse anti Sheep Interleukin-6 antibody, clone 4B6
货号：MCA1659
规格：0.25 mg
价格：5670
产品类型：流式抗体
品牌：Bio-Rad
抗原：IL-6
物种：绵羊
宿主：小鼠
抗体亚型：IgG1
克隆号：4B6
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Sheep Interleukin-6 antibody, clone 4B6recognizes ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells. Mouse anti Sheep Interleukin-6 antibody, clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha. |
| 产品详情 | | |
Target Species | Sheep |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Recombinant ovine IL-6. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry 1 | √ | | |
| ELISA | √ | | 5ug/ml (as a coating antibody) |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
- ELISAMCA1659 may also be used in combination withAHP424in a sandwich ELISA assays for ovine IL-6.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | Flow | 100 Tests |
| 文献 |
| 1. Herry, C.L.et al.(2016) Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.PLoS One. 11 (4): e0153515.2. Ciliberti, M.G.et al.(2017) Peripheral blood mononuclear cell proliferation and cytokine production in sheep as affected by cortisol level and duration of stress.J Dairy Sci. 100 (1): 750-756.3. Westover, A.et al.(2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.Front Physiol. 8: 871.4. Castel, A.et al.(2021) Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep.J Neurosci Methods. 360: 109257.5. Ciliberti, M.G.et al.(2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation usingin vitro.sheep model.J Dairy Sci. Mar 17 [Epub ahead of print].6. Cao, M.et al. (2022) The vagus nerve regulates immunometabolic homeostasis in the ovine fetus near term: impact on terminal ileumasXiv 2203.149677. Damiano, S.et al.(2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambsIt J anim Sci 21 (1): 934-42. |
技术参数 产品应用 ELISA;WB;Flow
产品类型 Monoclonal Antibody
推荐用于 ELISA;WB;Flow
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