| 产品介绍 | | |
| Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4recognizes ovine interleukin-1 beta, and shows no cross-reactivity with ovine IL-6, IL-8, TNF alpha or MCP-1.Mouse anti Sheep Interleukin-1 beta antibody, clone 1D4 demonstrates partial neutralizing activity of ovine IL-1 beta. |
| 产品详情 | | |
Target Species | Sheep |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Goat | √ |
| Bovine | √ |
| Horse | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
BufferSolution | Phosphate buffered saline |
Immunogen | Recombinant ovine IL-1 Beta |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry 1 | √ | | 1/10 |
| ELISA | √ | | |
| Immunohistology - Frozen | √ | | |
| Western Blotting | X | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product CodeBUF09) for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
- ELISAMouse anti Interleukin-1 beta antibody, clone 1D4 may be used as a capture antibody in a bovine IL-1 beta sandwich ELISA together with Rabbit anti Bovine interleukin-1β antibody (AHP851B) as the detection reagent for evaluation of IL-1β levels in bovine samples together with recombinant Bovine interleukin-1β (PBP008) used as standards.Alternatively, Mouse anti Interleukin-1 beta antibody, clone 1D4 can be used as a capture reagent together with Rabbit anti Ovine interleukin-1β antibody (AHP423) as a detection reagent for the evaluation of IL-1β levels in ovine, bovine or caprine samples, again utilizing recombinant bovine IL-1&beta (PBP008) as an internal standard.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | Flow | 100 Tests |
| 文献 |
| 1. Doull, L.et al.(2015) Late production of CXCL8 in ruminant oro-nasal turbinate cells in response toChlamydia abortusinfection.Vet Immunol Immunopathol. 168 (1-2): 97-102.2. Xu, A.et al.(2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.Reprod Sci. 22 (11): 1409-20.3. Sobotta, K.et al.(2016)Coxiella burnetii.Infects Primary Bovine Macrophages and Limits Their Host Cell Response.Infect Immun. 84 (6): 1722-34.4. Cortes, M.et al.(2017) RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: A glialin vivo-in vitromulti-hit paradigm in large mammalian brain.J Neurosci Methods. 276: 23-32.5. Canal AMet al.(2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with naturalMycobacterium bovisinfection.Res Vet Sci. 110: 34-39.6. Cao, M.et al.(2019) α7 Nicotinic Acetylcholine Receptor Signaling Modulates Ovine Fetal Brain Astrocytes Transcriptome in Response to Endotoxin.Front Immunol. 10: 1063.7. Stassi, A.F.et al.(2019) Follicular structures of cows with cystic ovarian disease present altered expression of cytokines.Zygote. 15: 1-14.8. Ciliberti, M.G.et al.(2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation usingin vitro.sheep model.J Dairy Sci. Mar 17 [Epub ahead of print].9. Damiano, S.et al.(2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambsIt J Anim Sci. 21 (1): 934-42. |
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