| 产品介绍 | | |
| Mouse anti Human CD49f antibody, clone 450-30Arecognizes the human VLA-6 cell surface antigen, also known as the alpha 6 integrin and as CD49f.CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes.CD49f is also widely expressed on epithelial tissues. |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1 ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose |
| Immunogen | alpha 6 beta 4 integrin purified from A431 cells. |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 数据 |
| Staining of human peripheral blood monocytes with RPE conjugated Mouse anti Human CD49f antibody, clone 450-30A (MCA1457PE). |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE | MCA928PE | Flow | 100 Tests |
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| 文献 |
1. Cavers, M.et al.(2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.Clin Exp Immunol. 127: 60-5.2. López, J.et al.(2012) Cancer-initiating cells derived from established cervical cell lines exhibit stem-cell markers and increased radioresistance.BMC Cancer. 12: 48.3. Keller, P.J.et al.(2012) Defining the cellular precursors to human breast cancer.Proc Natl Acad Sci U S A. 109: 2772-7.4. Kaczmarek, M.et al.(2011) Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions.Tumour Biol. 32: 1123-32.5. Aldridge, V.et al.(2012) Human mesenchymal stem cells are recruited to injured liver in a β1-integrin and CD44 dependent manner.Hepatology. 56 (3): 1063-73.6. Steinberg, F.et al.(2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.J Cell Biol. 197 (2): 219-30.7. Liu, L.et al.(2003) Priming of eosinophil migration across lung epithelial cell monolayers and upregulation of CD11b/CD18 are elicited by extracellular Ca2+.Am J Respir Cell Mol Biol. 28: 713-21.8. Goyer, B.et al.(2017) Extracellular matrix and integrin expression profiles in Fuchs endothelial corneal dystrophy cells and tissue model.Tissue Eng Part A. Jul 20 [Epub ahead of print].
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