| 产品介绍 | | |
| Mouse anti PCNA antibody, clone PC10recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Liet al.1995) through its interaction withFEN1(Wuet al.1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravoet al.1987).PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Parket al.2008), chicken (Franz-Odendaal 2008) and abalone (Harriset al.2006). |
| 产品详情 | | |
Target Species | Rat |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Vertebrates | Expected from Sequence |
| Invertebrates | Expected from Sequence |
| Ferret | √ |
| Chicken | √ |
| Rabbit | √ |
Xenopus | √ |
| Mouse | √ |
| Horse | √ |
| Sheep | √ |
| Dog | √ |
| Cat | √ |
| Cynomolgus monkey | √ |
| Rhesus Monkey | √ |
| Hamster | √ |
| Atlantic Salmon | √ |
Human | √ |
| Bearded Dragon | √ |
| Corn Snake | √ |
| Nile Crocodile | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.01% Thiomersal HRP Stabiliser (BUF052A) |
| Immunogen | Rat PCNA made in the protein A expression vector pR1T2T |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| WB | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Fenton, M.et al.(2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.Arterioscler Thromb Vasc Biol. 21: 220-6.2. Park, J.H.et al.(2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.J Comp Pathol. 139: 208-17.3. Izhak, L.et al.(2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.PLoS One. 7: e28305.4. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.Sci Adv. 2 (6): e1600708.5. Guzera, M.et al.(2016)In VitroInfluence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.PLoS One. 11 (5): e0154429.6. Khlebnikov, V. V.et al.(2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical StressNeuroscience and Behavioral Physiology. 46 (1): 100-5.7. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.J Clin Pediatr Dent. 42 (5): 391-7.8. Falbo, L.et al.(2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.Nat Commun. 11 (1): 1345. |
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