| 产品介绍 | | |
| Mouse anti Human Interferon gamma antibody, clone B27recognizes human interferon gamma (IFN gamma). IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.Mouse anti Human Interferon gamma antibody, clone B27 is reported to neutralize the antiviral activity of human IFN gamma (Favreet al.1989). |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide 1%Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
| Immunogen | Recombinant human IFN gamma. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry1 | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:FITC | MCA928F | Flow | 100 Tests |
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| Human Seroblock | BUF070A | Flow | 50 Tests |
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| 数据 |
| Figure A. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Rat IgG2a (MCA6005PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A)for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Mouse anti Human IL-2 (MCA1553PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer |
| 文献 |
1. Favre, C.et al.(1989) Epitope mapping of recombinant human gamma interferon using monoclonal antibodies.Mol Immunol. 26 (1): 17-25.2. Abrams, J.S.et al.(1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.Immunol Rev. 127: 5-24.3. Paliard, X.et al.(1988) Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones.J Immunol. 141 (3): 849-55.4. Gomes, J.A.et al.(2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.Infect Immun. 71 (3): 1185-93.5. Lai, X.et al.(2003) Immune biology of macaque lymphocyte populations during mycobacterial infection.Clin Exp Immunol. 133: 182-92.6. Wakkach, A.et al.(2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.J Immunol. 167: 3107-13.
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