Rat anti mouse CD134:FITC
货号：MCA1420F,MCA1420FA,MCA1420FB
规格：0.1 mg,50 µg,0.5 mg
价格：2576,1513,5143
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD134
物种：小鼠
宿主：大鼠
抗体亚型：IgG1
克隆号：OX-86
荧光染料：FITC
| 产品介绍 | | |
| Rat anti Mouse CD134 antibody, clone OX-86recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin |
| Immunogen | CHO cells transfected with murine OX40. |
| Approx. Protein Concentrations | Pack Size: 0.1 mg IgG concentration 0.1 mg/ml Pack Size: 0.5 mg IgG concentration 0.5 mg/ml |
Fusion Partners | Spleen cells from immunized AO rats were fused with cells of the mouse NS1 myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 1 x106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). |
| 同型对照 |
| Description | Product Code | Applications | Pack Size |
| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | Flow | 0.1mg |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
| 文献 |
| 1. Maenz, M.et al.(2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.Mol Vis. 17: 420-9.2. Zaini, J.et al.(2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.J Clin Invest. 117: 3330-8.3. Andarini, S.et al.(2004) Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.Cancer Res. 64: 3281-7.4. Malmström, V.et al.(2001) CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.J Immunol. 166: 6972-81.5. McHugh, R.S.et al.(2002) CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor.Immunity. 16: 311-23.6. Thaunat, O.et al.(2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.PLoS One. 5: e11398.7. Wythe, S.E.et al.(2012) OX40 Ligand and Programmed Cell Death 1 Ligand 2 Expression on Inflammatory Dendritic Cells Regulates CD4 T Cell Cytokine Production in the Lung during Viral DiseaseJ Immunol. 188: 1647-55.8. Reynolds, C.et al.(2014) Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.BMC Biol. 12: 32. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
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