mouse anti human AGGRECAN
货号：MCA1454G
规格：0.2 mg
价格：3315
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：AGGRECAN
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：7D4
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human Aggrecan antibody, clone 7D4recognizes human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS).Using a panel of core protein-directed antibodies directed against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. highlighting a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintinoet al.2009). Mouse anti Human Aggrecan antibody, clone 7D4 recognizes epitopes within the N-terminal G1-IGD-G2 region of aggrecan, identified as a cluster 1 isoform. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Bovine | √ |
| Chicken | X |
| Fish | X |
| Rat | X |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Immunogen | Purified human articular cartilage aggrecan. |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| Immunocytochemistry | √ | | |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
1. Betre, H.et al.(2002) A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineeringEngineering in Medicine and Biology 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference,: 829-30.2. Shintani Net al.(2013) TGF-β1 enhances the BMP-2-induced chondrogenesis of bovine synovial explants and arrests downstream differentiation at an early stage of hypertrophy.PLoS One. 8 (1): e53086.3. Blosa, M.et al.(2013) Unique features of extracellular matrix in the mouse medial nucleus of trapezoid body--implications for physiological functions.Neuroscience. 228: 215-34.4. Wan, S.et al.(2016) Self-assembling peptide hydrogel for intervertebral disc tissue engineering.Acta Biomater. 46: 29-40.5. Rogers, S.L.et al.(2018) Normal Development of the Perineuronal Net in Humans; In Patients with and without Epilepsy.Neuroscience. 384: 350-60.6. Goldberg-Bockhorn, E.et al.(2018) Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering.Lasers Med Sci. 33 (2): 375-84.7. De Moor, L.et al.(2020) Scaffold Free Microtissue Formation for Enhanced Cartilage Repair.Ann Biomed Eng. 48 (1): 298-311.
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技术参数 产品应用 IHC;ELISA;WB;IP
产品类型 Monoclonal Antibody
推荐用于 IHC;ELISA;WB;IP
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