| 产品介绍 | | |
| Mouse anti V5-Tag, clone SV5-Pk1recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunnet al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randallet al.1993andZhaoet al.2005). |
| 产品详情 | | |
Target Species | Viral |
| Product Form | Purified IgG conjugated to Alkaline Phosphatase - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Paramyxovirus Simian-Virus 5 (SV5) |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| Western Blotting | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Kovács, H.A.et al. (2021) Characterization of the Proprotein Convertase-Mediated Processing of Peroxidasin and Peroxidasin-like ProteinAntioxidants. 10 (10): 1565.2. Lucas, R.M.et al.(2021) SCIMP is a spatiotemporal transmembrane scaffold for Erk1/2 to direct pro-inflammatory signaling in TLR-activated macrophages.Cell Rep. 36 (10): 109662.3. Kao, C.Y.et al.(2021) Evidence for complex dynamics during U2 snRNP selection of the intron branchpoint.Nucleic Acids Res. 49 (17): 9965-97.4. Forey, R.et al.(2021) A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.Mol Cell. 81 (1): 183-197.e6.4. Hassler, M.et al.(2019) Structural Basis of an Asymmetric Condensin ATPase Cycle.Mol Cell. 74 (6): 1175-1188.e9.5. Bracci, N.et al.(2022) Rift Valley fever virus Gn V5-epitope tagged virus enables identification of UBR4 as a Gn interacting protein that facilitates Rift Valley fever virus production.Virology. 567: 65-76.6. Wang, Y.T.et al.(2022) K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy.Cell Rep. 38 (8): 110354.7. Zhu, H.et al.(2022) A comprehensive temporal patterning gene network inDrosophila.medulla neuroblasts revealed by single-cell RNA sequencing.Nat Commun. 13 (1): 1247. |
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