Rat anti Mouse CD117 antibody, clone 2B8
货号：MCA1365G
规格：0.25 mg
价格：3043
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD117
物种：小鼠
宿主：大鼠
抗体亚型：IgG2b
克隆号：2B8
荧光染料：Purified
| 产品介绍 | | |
| Rat anti Mouse CD117 antibody, clone 2B8recognizes mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.Rat anti Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice,CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G. |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
| Carrier Free | Yes |
| Immunogen | IL-3 dependent mast cells. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/50 | 1/200 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells in 100ul. |
| 文献 |
| 1. Fleming WHet al.(1993) Steel factor influences the distribution and activity of murine hematopoietic stem cellsin vivo.Proc Natl Acad Sci U S A. 90 (8): 3760-4.2. Rico-Vargas, S.A.et al.(1994) c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody.J Immunol. 152 (6): 2845-52.3. Manova, K.et al.(1992) c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons.J Neurosci. 12 (12): 4663-76.4. Maeda, H.et al.(1992) Requirement of c-kit for development of intestinal pacemaker system.Development. 116 (2): 369-75.5. Takagi, M.et al.(1992) Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor.J Immunol. 148 (11): 3446-53.6. de Vries, P.et al.(1992) Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.J Exp Med. 176 (6): 1503-9. |
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