| 产品介绍 | | |
| Mouse anti Cat CD8 alpha/beta antibody, clone vpg9recognizes the feline homolog of human CD8. Clone vpg 9 specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (Shimojimaet al.1998). |
| 产品详情 | | |
Target Species | Cat |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Human | X |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
Reconstitution | Reconstitute with 1 ml distilled water |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin |
| Immunogen | Feline thymocytes. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunized BALB/c were fused with cells of the NS0 mouse myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | Neat |
| Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106lymphocytes in 100ul |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
| 数据 |
| Cat lymphocytes stained with Mouse anti Cat CD8:Alpha/Beta (MCA1347GA) followed by Goat anti Mouse IgG:FITC (STAR117F); dual stained with Mouse anti Cat CD4:RPE |
| 文献 |
| 1. Pistello, M.et al.(2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.J Virol. 84: 3845-56.2. Willett, B.J.et al.(2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.J Virol. 81: 9665-79.3. Willett, B.J.et al.(2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.PLoS One. 8 (1): e54871.4. Milner, R.J.et al.(2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.J S Afr Vet Assoc. 75 (2): 103-7.5. Rydzewski, L.et al.(2016) Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.Vet Immunol Immunopathol. 177: 24-34.6. de Oliveira Medeiros, S.et al.(2016) Follow-up on long-term antiretroviral therapy for cats infected with feline immunodeficiency virus.J Feline Med Surg. 18 (4): 264-72.7. Guzera, M.et al.(2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.Vet Comp Oncol. 14 Suppl 1: 40-51.8. O'Brien, T.et al.(2015) Moderate dietary supplementation with vitamin E enhances lymphocyte functionality in the adult cat.Res Vet Sci. 99: 63-9.9. Sparger, E.E.et al.(2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.Vet Immunol Immunopathol. 202: 52-62.10.Maeta, N.et al.(2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.Open Vet J. 9 (2): 147-50.11. Mustaffa-Kamal, F.et al.(2019) Characterization of antiviral T cell responses during primary and secondary challenge of laboratory cats with feline infectious peritonitis virus (FIPV).BMC Vet Res. 15 (1): 165. |
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