Mouse anti Rat IgG2c Heavy Chain: FITC
货号：MCA1295F
规格：0.5 mg
价格：7565
产品类型：流式抗体
品牌：Bio-Rad
抗原：IgG2c Heavy Chain
物种：大鼠
宿主：小鼠
抗体亚型：IgG2a
克隆号：MARG2c-5
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Rat IgG2c heavy chain antibody, clone MARG2c-5recognizes the heavy chain of rat immunoglobulin gamma 2C as determined by dot blot analysis. |
| 产品详情 | | |
Target Species | Rat |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3) 50% Glycerol |
| Immunogen | IgG2c (Rat strain LOU/c). |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | 5ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG2a Negative Control:FITC | MCA1210F | F | 0.1 mg |
| 文献 |
| 1. Manouvriez, P.et al. (1986) Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by ELISA with mouse monoclonal antibodies.Immunobiology 173: 307-8.2. Delriviere, L.D.et al.(1998) Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.Transplantation. 66: 112-5.3. Lin, Y.et al.(1999) Accommodated xenografts survive in the presence of anti-donor antibodies and complement that precipitate rejection of naive xenografts.J Immunol. 163: 2850-7.4. Lin, Y.et al.(2000) Long-term survival of hamster hearts in presensitized rats.J Immunol. 164: 4883-92.5. Ueta, H.et al.(2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.Int Immunol. 30 (2): 53-67.Further Reading1. van den Berg, T.K.et al.(2001) Monoclonal antibodies against rat leukocyte surface antigens.Immunol Rev. 184: 109-16. |
技术参数 产品应用 Flow
产品类型 单克隆抗体
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