Rat anti Mouse Kappa Light Chain:HRP
货号:MCA1291P
规格:0.5 mg
价格:7973
产品类型:免疫组化一抗
品牌:Bio-Rad
抗原:Kappa Light Chain
物种:小鼠
宿主:大鼠
抗体亚型:IgG2a
克隆号:LO-MK-1
荧光染料:HRP
产品介绍 | | |
Rat anti Mouse kappa light chain antibody, clone LO-MK-1recognises the murine kappa immunoglobulin light chain, and does not cross-react murine immunoglobulin heavy chains or lambda light chains. |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid |
Preparation | Purified IgG prepared by affinity chromatography from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.01% Thiomersal 50% Glycerol |
Immunogen | Purified mouse IgG1 kappa. |
Approx. Protein Concentrations | IgG concentration 1 mg/ml |
Fusion Partners | Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 500ng/ml | 1000ng/ml |
Immunohistology - Frozen | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together withMCA1589as the capture reagent. |
相关产品 |
Description | Product Code | Applications | Pack Size |
AbGUARD® HRP Stabilizer Plus | BUF052A | ELISA WB IHC(P)IHC(P) | 100 ml |
AbGUARD® HRP Stabilizer Plus | BUF052B | ELISA WB IHC(P)IHC(P) | 500 ml |
AbGUARD® HRP Stabilizer Plus | BUF052C | ELISA WB IHC(P)IHC(P) | 1000 ml |
TMB Signal+ | BUF054A | ELISA | 100 ml |
TMB Core | BUF056A | ELISA | 100 ml |
TMB Core+ | BUF062A | ELISA | 100 ml |
文献 |
1. Delaunay, T.et al.(1990) Rat (and mouse) monoclonal antibodies. VIII. ELISA measurement of Ig production in mouse hybridoma culture supernatants.J Immunol Methods. 131 (1): 33-9.2. Savignac. M.et al.(2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.J Immunol. 185:7527-36.3. Laffont Set al.(2017) Androgen signaling negatively controls group 2 innate lymphoid cells.J Exp Med. pii: jem.20161807. [Epub ahead of print] |
技术参数 产品应用 C ;E
产品类型 单克隆抗体
推荐用于 C ;E
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