| 产品介绍 | | |
| Rat anti Mouse IgG1 antibody, clone LO-MG1-13recognizes the gamma 1 heavy chain of mouse immunoglobulin. No cross reactivity with other immunoglobulin classes or subclasses. |
| 产品详情 | | |
Target Species | Mouse |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Human | X |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3) |
| Immunogen | Purified mouse IgG1 from BALB/c mice. |
| Approx. Protein Concentrations | IgG concentration 1 mg/ml |
Fusion Partners | Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 5ug/ml as coating antibody |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.This antibody may be used as a coating antibody in a sandwich ELISA in combination with LO-MG1-2 (MCA336P) as the detection reagent. |
| 文献 |
| 1. Denis, O.et al.(1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.Int Immunol. 5 (1): 71-8.2. Soares, M.et al.(1992) Isotype characterization of rat preformed natural antibodies against guinea pig cells.Transplant Proc. 24 (2): 451-2.3. Savignac. M.et al.(2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.J Immunol. 185:7527-36.4. Banerjee, K.et al.(2012) Occluding the mannose moieties on human immunodeficiency virus type 1 gp120 with griffithsin improves the antibody responses to both proteins in mice.AIDS Res Hum Retroviruses. 28 (2): 206-14.5. Laffont Set al.(2017) Androgen signaling negatively controls group 2 innate lymphoid cells.J Exp Med. pii: jem.20161807. [Epub ahead of print] |
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