| 产品介绍 | | |
| Rat anti FITC antibody, clone LO-FLUO-1reacts with fluorescein isothiocyanate. Avidity against FITC conjugated BSA is 2 x 1010M-1.This antibody binds to both Free FITC and FITC conjugated to proteins, and may be valuable for amplification of FITC based immunostaining. |
| 产品详情 | | |
Target Species | Chemical |
| Product Form | Purified IgM - liquid |
Preparation | Purified IgM prepared by affinity chromatography from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 50%Glycerol 0.1%Sodium Azide |
| Immunogen | FITC conjugated mouse immunoglobulin. |
| Approx. Protein Concentrations | Isotype concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
| ELISA | √ | | 0.5ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Van Cauwenberge, A.et al.(2009) Development of a new low-cost and regenerable detection device for microbial compounds MIC-ATRFinal Report Phase 1 Summary Brussels : Belgian Science Policy 2009 – 6 p. (Research Programme Science for a Sustainable Development) . |
QQ:1749072012