| 产品介绍 | | |
| Mouse anti Human CD100 antibody, clone A8recognizes human Sepaphorin 4D, also known as CD100, A8, BB18 or GR3. CD100 is a 841 amino acid ~150 kDa homodimeric single pass type 1 transmembrane cell surface glycoprotein acting as a receptor for some members of the plexin family. CD100 is expressed at low levels by peripheral blood T cells, and at higher density on activated T cells and B cells, it is also expresses at high levels in fetal kidney with lower levels in fetal brain, liver and lung (Maestriniet al.1996). |
| 产品详情 | | |
Target Species | Human |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Cynomolgus monkey | √ |
| Rhesus Monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Allophycocyanin (APC) - lyophilized |
Reconstitution | Reconstitute with 1ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin 5%Sucrose |
| Immunogen | Human PHA activated lymphocytes. |
Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
APC | 650 | 661 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells in 100ul. |
| 数据 |
| Staining of human peripheral blood lymphocytes with Mouse anti Human CD100:APC (MCA1269APC). |
| 同型 |
| Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
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| 文献 |
| 1. Aversa, G.G.et al.(1989) Use of monoclonal antibodies to studyin vivoandin vitro-activated lymphocytes.Transplant Proc. 21 (1 Pt 1): 349-50.2. Van Vliet, S. J.et al. (1995) Species cross reactivity (human-monkey-bovine) of the adhesion structure section mAb.Leucocyte Typing V. Oxford University Press p1607-8.3. Mizrahi, S.et al.(2007) CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.PLoS One. 2 (9): e818.4. Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110. |
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