Rat anti Dog CD45R antibody, clone YKIX753.22.2
货号:MCA1043PE
规格:100 Tests
价格:4250
产品类型:流式抗体
品牌:Bio-Rad
抗原:CD45R
物种:dog
宿主:大鼠
抗体亚型:IgG2b
克隆号:YKIX753.22.2
荧光染料:PE
产品介绍 | | |
Rat anti Dog CD45R antibody, clone YKIX753.22.2recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts, (Cobboldet al.1994). It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibodyclone YKIX716.13(Cobboldet al.1994). |
产品详情 | | |
Target Species | Dog |
Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
Reconstitution | Reconstitute with 1ml distilled water |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
Immunogen | Canine thymocytes |
Approx. Protein Concentrations | IgG concentration 0.05mg/ml |
Fusion Partners | Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
RPE 488nm laser | 496 | 578 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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Description | Product Code | Applications | Pack Size |
Rat IgG2b Negative Control:RPE | MCA6006PE | F | 100 Tests |
文献 |
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54. |
技术参数 产品应用 Flow
产品类型 单克隆抗体
推荐用于 Flow
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