Rat anti Dog CD45 antibody, clone YKIX716.13
货号：MCA1042APC
规格：100 Tests
价格：4293
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD45
物种：dog
宿主：大鼠
抗体亚型：IgG2b
克隆号：YKIX716.13
荧光染料：Alexa Fluor 488
| 产品介绍 | | |
| Rat anti Dog CD45 antibody, clone YKIX716.13recognizes canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop (CLAW). Clone YKIX 716.13: immunoprecipitates an antigen of ~180/200 kDa from Con-A blasts (Cobboldet al.1994). CD45 is expressed on all leukocytes in canine peripheral blood. Rat anti Dog CD45 antibody, clone YKIX716.13 reacts with CD45 on all outbred mongrels and beagles tested and may be against CD45RB isoform. |
| 产品详情 | | |
| Target Species | Dog |
| Product Form | Purified IgG conjugated to Allophycocyanin (APC) - lyophilized |
| Reconstitution | Reconstitute with 1ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide1%Bovine Serum Albumin |
| Immunogen | Canine thymocytes. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| APC | 650 | 661 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
| 产品推荐 |
| Description | Product Code | Applications | Pack Size |
| Rat IgG2b Negative Control:APC | MCA6006APC | F | 100 Tests |
| 文献 |
| 1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.2.Gorman, S.D.et al.(1994) Isolation and expression of cDNA encoding the canine CD4 and CD8 alpha antigens.Tissue Antigens. 43 (3): 184-8.3.Watson, C.J.et al.(1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog.Br J Surg. 80 (11): 1389-92. |
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