Mouse anti Mouse CD90 antibody, clone F7D5
货号:MCA02RT,MCA02R
规格:25 µg,0.25 mg
价格:808,3018
产品类型:流式抗体
品牌:Bio-Rad
抗原:CD90
物种:小鼠
宿主:小鼠
抗体亚型:IgM
克隆号:F7D5
荧光染料:Purified
产品介绍 | | |
Mouse anti Mouse CD90 antibody, clone F7D5recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lakeet al.1979).Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes. |
产品详情 | | |
Target Species | Mouse |
Product Form | IgM fraction - liquid |
Preparation | IgM fraction prepared by ammonium sulphate precipitation |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Fusion Partners | Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line. |
Approx. Protein Concentrations | IgM concentration 1.0 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Cytotoxic Assays | √ | | |
Flow Cytometry | √ | 1/10 | 1/25 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
二抗 |
Description | Product Code | Applications | Pack Size |
Human anti Mouse IgM:FITC | HCA040F | F | 0.1 mg |
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | E,WB | 1 ml |
文献 |
1.Lake, P.et al.(1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.Eur J Immunol. 9 (11): 875-86.2.Yoshida, K.et al.(2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.Int Immunol. 14 (12): 1439-47.3.DeVries-vanDerZwan, A.et al.(1997) Specific tolerance induction and transplantation: a single-day protocol.Blood. 89 (7): 2596-601.4.Winzeler, A.M.et al.(2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.J Neurosci. 31: 6481-92. |
技术参数 产品应用 flow
产品类型 单克隆抗体
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