| 产品介绍 | | |
| Mouse anti Mouse CD90 antibody, clone F7D5recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lakeet al.1979).Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | IgM fraction conjugated to Pacific Blue - liquid |
| Preparation | IgM fraction prepared by ammonium sulfate precipitation |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Fusion Partners | Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Pacific Blue® | 410 | 455 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Approx. Protein Concentrations | IgM concentration 0.05 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Lake, P.et al.(1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.Eur J Immunol. 9 (11): 875-86.2.Yoshida, K.et al.(2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.Int Immunol. 14 (12): 1439-47.3.DeVries-vanDerZwan, A.et al.(1997) Specific tolerance induction and transplantation: a single-day protocol.Blood. 89 (7): 2596-601.4.Winzeler, A.M.et al.(2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.J Neurosci. 31: 6481-92.5.Unterlauft, J.D.et al.(2014) Enhanced survival of retinal ganglion cells is mediated by Müller glial cell-derived PEDF.Exp Eye Res. 127: 206-14. |
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