| 产品介绍 | | |
| LYNX Rapid Plus Biotin (Type 1) Antibody Conjugation Kit®enables the rapid conjugation of a pre-prepared lyophilized mixture containing Biotin label to an antibody or protein in minutes. Activation of proprietary reagents within the antibody-label solution results in the coupling of the antibody to Biotin.The LYNX Rapid Plus Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery and no requirement for desalting or dialysis.This kit has been optimized for the additional use of a streptavidin labelled reagent. For customers intending to capture their conjugate on Streptavidin coated plates, we recommend ourLYNX Rapid Plus Biotin (Type 2) Antibody Conjugation Kitinstead. |
| 产品详情 | | |
| Reagents in the Kit | Pack Size: 3 Conjugations For 20µg Antibody3 Vials of 10ug LYNX Rapid Plus lyophilized Biotin mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 3 Conjugations For 200µg Antibody3 Vials of 100ug LYNX Rapid Plus lyophilized Biotin mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 1 Conjugation For 2mg Antibody1 Vial of 1mg LYNX Rapid Plus lyophilized Biotin mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagent |
| Preparing The Antibody | Pack Size: 3 Conjugations For 20µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.Do not use buffers containing nucleophilic components (e.g primary amines), thiols (e.g. Thiomersal/Thimerosal), Merthiolate, Glycine or Proclin. These substances may react with LYNX chemicals.Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.It is recommended that up to 10-20 μg antibody be used in each labelling reaction. For optimal results the antibody volume should be 4-10 μl, at a concentration range between 1mg/ml and 2.5 mg/ml.Pack Size: 3 Conjugations For 200µg AntibodyThe following buffer solutions are recommended:for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.Do not use buffers containing nucleophilic components (e.g primary amines), thiols (e.g. Thiomersal/Thimerosal), Merthiolate, Glycine or Proclin. These substances may react with LYNX chemicals.Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.It is recommended that up to 100-200 μg antibody be used in each labeling reaction. For optimal results the antibody volume should be 40-100 μl, at a concentration range between 1mg/ml and 2.5 mg/ml.Pack Size: 1 Conjugation For 2mg AntibodyThe following buffer solutions are recommended:for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.Do not use buffers containing nucleophilic components (e.g primary amines), thiols (e.g. Thiomersal/Thimerosal), Merthiolate, Glycine or Proclin. These substances may react with LYNX chemicals.Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.It is recommended that up to 2mg antibody be used in each labeling reaction. For optimal results the antibody volume should be 400-1000ul, at a concentration range between 1mg/ml and 2.5 mg/ml. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This kit contains lyophilized hygroscopic components that are moisture-sensitive. This kit is shipped under ambient conditions with silica packets to avoid exposure to moisture. On receipt, Bio-Rad recommend that the kit is stored at -20℃ and protected from moisture. Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing. Before opening, allow the components to reach room temperature to minimize condensation. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Conjugation | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.- Instructions For Use
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25°C) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
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