Human anti Bovine Interleukin-2, clone AbD22297
货号：HCA268P
规格：0.1 mg
价格：3638
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Bovine Interleukin-2
物种：人
宿主：人
抗体亚型：其它
克隆号：AbD22297
荧光染料：HRP
| 产品介绍 | | |
| Human anti Bovine Interleukin-2, clone AbD22297recognizes bovine interleukin-2 (IL-2), also known as T-cell growth factor (TCGF). Bovine IL-2 is a 155 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.Human anti bovine interleukin-2, clone AbD22297, has been successfully used for the detection and measurement of native and recombinant bovine IL-2 in ELISA where it acts as the detection antibody in a sensitive assay for bovine IL-2 determination. Bio-Rad recommend clone AbD21766 (HCA267) for intracellular flow cytometry. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed inE. coli.This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a V5 tag and a double extended Strep-tag (SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK). |
| Preparation | Metal chelate affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | Fc-fusion protein containing the amino acid sequence 21-155 from bovine interleukin-2 |
| Approx. Protein ConcePurityntrations | Antibody concentration 0.1 mg/ml |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped frozen.When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| ELISpot | √ | | |
| Flow Cytometry | × | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Mouse anti V5-Tag:HRP | MCA1360P | E，WB | 0.1mg |
| 文献 |
| 1.Rhodes, S.G.et al.(2014) Use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with Mycobacterium bovis BCG and cattle infected with M. bovis.Clin Vaccine Immunol. 21:39-45. |
技术参数 产品应用 elisa
产品类型 单克隆抗体
推荐用于 elisa
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