| 产品介绍 | | |
| Human anti Human HbA1c antibody, clone AbD15792recognizes HbA1c, i.e. hemoglobin when glycated at the N-terminal valine of the hemoglobin β chain. It does not bind to non-glycated hemoglobin. Besides binding to the normal glycated hemoglobin, it also recognizes the glycated hemoglobin variants HbD and HbE, but does not recognize the HbS or HbC variants. Elevated HbF cannot be detected due to the absence of the hemoglobin β chain.The measurement of HbA1c levels is used to identify the average plasma glucose concentration. In diabetes mellitus, higher levels of HbA1c have been associated with cardiovascular and other diseases. Levels of more than 6.5% HbA1c (48 mmol/mol) are used as a criterion to diagnose diabetes. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed inE. coli.The antibody is tagged with a V5 tag and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid. |
| Preparation | StrepTactin affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.01% Thiomersal |
| Immunogen | The immunogens used in the generation of this antibody were:A glycated peptide, corresponding to N-terminal 9 amino acids of haemoglobin beta chain coupled to carrier proteinsPurified HbA1cAlemtuzumab. |
| Affinity | The affinity of this antibody, as measured against the immunogen, was measured as KD=7 nM by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.The affinity of this antibody, as measured against purified HbA1c, was measured as KD=54 nM by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen. |
| Approx. Protein ConcePurityntrations | Total protein concentration 0.5 mg/ml |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 产品推荐 |
| Description | Product Code | Applications | Pack Size |
| Mouse anti V5-Tag:Alk. Phos. | MCA1360A | E | 0.1mg |
QQ:1749072012