Rabbit anti Human NFkB p65 antibody
货号：AHP288
规格：0.1 ml
价格：6800
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：NFkB p65
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human NFkB p65 antibodyrecognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFκB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFκB p65 is a 551 amino acid ~65 kDa subunit of the NFκB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFκB appears to be the p65-p50 heterodimer. In the active form NFκB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NFκB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IκB (UniProt: Q04206)In a gel supershift assay Rabbit anti Human NFκB p65 antibody was found to be active against all p65 containing human, mouse or rat NFκB complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti Human NFκB p65 antibody to the NFκB p65 subunit can be blocked using the NFκB p65 control peptide (PHP073). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Serum - liquid |
| Buffer Solution | Phosphate buffered saline |
| Antiserum Preparation | Antisera to NFkB p65 were raised by repeated immunisation of rabbits with highly purified antigen. |
| Immunogen | NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to KLH. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/5000 | 1/25000 |
| Western Blotting | √ | 1/2000 | 1/5000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Cruz, M.T.et al.(1999) Involvement of JAK2 and MAPK on type II nitric oxide synthase expression in skin-derived dendritic cells.Am J Physiol. 277: C1050-7.2.Parmentier. M,et al. (2000) Regulation of lipopolysaccharide-mediated interleukin-1beta release by N-acetylcysteine in THP-1 cells.Eur. Respir. J. 16: 933-9.3.Gründker, C.et al.(2000) Luteinizing hormone-releasing hormone induces nuclear factor kappaB-activation and inhibits apoptosis in ovarian cancer cells.J Clin Endocrinol Metab. 85: 3815-20.4.Sun, J.et al.(2007) Neuropeptide substance P upregulates chemokine and chemokine receptor expression in primary mouse neutrophils.Am J Physiol Cell Physiol. 293: C696-704.5.Wang, J.et al.(2014) TL1-A can engage death receptor-3 and activate NF-kappa B in endothelial cells.BMC Nephrol. 15: 178. |
技术参数 产品应用 WB
产品类型 Polyclonal Antibody
推荐用于 WB
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