| 产品介绍 | | |
| Rabbit anti Human Interleukin-7 antibodyrecognizes human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide. |
| Buffer Solution | Phosphate buffered saline |
| Antiserum Preparation | Antisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography. |
| Preservative Stabilisers | Phosphate buffered saline |
| Immunogen | Recombinant human IL-7 (PHP046). |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 | 2.0 ug/ml |
| Functional Assays | √ | 0.-5 | 0.08 ug/ml |
| Western Blotting | √ | 0.1 | 0.2 ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Lupton, S.D.et al.(1990) Characterization of the human and murine IL-7 genes.J Immunol. 144 (9): 3592-601.2.Fry, T.J. & Mackall, C.L. (2002) Interleukin-7: from bench to clinic.Blood. 99 (11): 3892-904. |
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