Rabbit anti caspase-3 antibody
货号：AHP2717
规格：50 µl
价格：2576
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：caspase-3
物种：其它
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti caspase-3 antibodyrecognizes caspase-3 also known as cysteine protease CPP32. Caspase-3, as the name implies, is a member of the cysteine-aspartic acid protease (caspase) protein family.Caspase-3 is an effector caspase that is activated by both the intrinsic and extrinsic apoptosis pathways. Caspase-3 is cleaved from an inactive 32 kDa zymogen/pro-enzyme into p17 and p12 subunits by the activated initiator caspases 9 and 8 in apoptotic cells. These subunits dimerize to form the active caspase 3 protein that cleaves and activates caspase-6 and 2. Through the activation/cleavage of downstream caspases, caspase-3 plays an important role in mediating apoptosis signaling. |
| 产品详情 | | |
| Target Species | Human |
| Western Blotting | Rabbit anti caspase-3 antibody detects a band of approximately 32 kDa in Jurkat cell lysate under reducing conditions |
| Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antiserum to caspase-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
| Immunogen | Recombinant human caspase-3 |
| Regulatory | For research purposes only |
| Acknowledgements | PrecisionAb is a trademark of Bio-Rad Laboratories |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/500 | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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