Rabbit anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) antibody
货号：AHP2700
规格：50 µl
价格：3341
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425)
物种：其它
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) antibodyrecognizes mothers against decapentaplegic homolog 2 (SMAD2), when phosphorylated at serine 465 and serine 467 and mothers against decapentaplegic homolog 3 (SMAD3) when phosphorylated at serine 423 and serine 425. The SMAD protein family mediates transforming growth factor-beta (TGF-β) signaling from the membrane to the nucleus (Attisano, L. & Wrana, J.L. 2002). SMAD2 is phosphorylated on serine 465 and serine 467 by TGF beta type I receptor (TbetaRI). This phosphorylation is essential for SMAD2’s binding to SMAD4 and for the orchestration of TGF beta signaling (Abdollah, S. et al. 1997). The antibody also recognizes SMAD3 phosphorylated on Ser423 and Ser425 by homology.The TGF beta signaling pathway is involved in many cellular processes including cell growth, differentiation and proliferation. The pathway is deregulated in various diseases, including cancer. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antiserum to SMAD2 (pSer465/pSer467)/SMAD3 (pSer423/pSer425) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
| Immunogen | Phospho specific-peptide corresponding to residues surrounding serine 465/467 of human SMAD2 and serine 423/425 of human SMAD3 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/500 | 1/2000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| 文献 |
| 1.Ramsauer, K.et al.(2002) p38 MAPK enhances STAT1-dependent transcription independently of Ser-727 phosphorylation.Proc Natl Acad Sci U S A. 99 (20): 12859-64. |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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