Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody
货号：AHP2693
规格：50 µl
价格：3213
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr
物种：其它
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibodyrecognizes mitogen activated protein kinase (MAPK) 8 and 9, also known as c-Jun N-terminal kinases 1 and 2 or stress activated protein kinases 1 and 2, when phosphorylated on threonine 183 and tyrosine 185. This antibody also recognizes MAPK10, also known as c-Jun N-terminal kinase 3 or stress activated protein kinase 3, when phosphorylated on threonine 221 and tyrosine 223.As their name implies, MAPK8/9/10 are members of the serine/threonine MAP kinase family, which become activated by environmental stressors. In addition, activation through phosphorylation events has been reported upon stimulation of toll-like receptors and exposure to pro-inflammatory cytokines (Guma and Firestein 2012,Bogoyevitch and Kobe 2006).Two phosphorylation events are critical for the activation of the three MAPKs. The first is on threonine 183, or in the case of MAPK10 threonine 221, by dual specificity mitogen-activated kinase kinase 7 (MKK7) and the second is on tyrosine 185, or in the case of MAPK10 tyrosine 223, by MKK4 (Fleming 2000). The phosphorylation undertaken by MKK4 and MKK7 is counteracted by phosphatases of the MAP kinase phosphatase (MKP) family (Guma and Firestein 2012).The three MAPKs play a critical role in mediating cell signaling cascades by phosphorylating transcription factors, such as c-Jun and p53, thereby regulating cellular processes including proliferation and apoptosis (Guma and Firestein 2012). |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antiserum to MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
| Immunogen | Phospho specific-peptide corresponding to residues surrounding threonine 183/tyrosine 185 of human MAPK8/MAPK9 and to residues surrounding threonine 221/tyrosine 223 of human MAPK10. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/500 | 1/2000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| 文献 |
| 1.Guma M & Firestein GS (2012) c-Jun N-Terminal Kinase in Inflammation and Rheumatic Diseases.Open Rheumatol J. 6: 220-31.2.Bogoyevitch MA & Kobe B (2006) Uses for JNK: the many and varied substrates of the c-Jun N-terminal kinases.Microbiol Mol Biol Rev. 70 (4): 1061-95.3.Fleming Yet al.(2000) Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7.Biochem J. 352 Pt 1: 145-54. |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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