Rabbit anti STAT3 (pSer727) antibody
货号：AHP2685
规格：50 µl
价格：3825
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：STAT3 (pSer727)
物种：其它
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti STAT3 (pSer727) antibodyrecognizes signal transducer and activator of transcription 3 (STAT3), also known as acute-phase response factor, when phosphorylated at serine 727. STAT3 is a member of the STAT family of transcription factors, playing a key role in numerous processes including cell differentiation, proliferation and transformation. Phosphorylation of STAT3 at tyrosine 705 leads to homo- or heterodimerization of STAT3 and is a critical phosphorylation site for STAT3 activity (Huanget al.2014).Serine 727 is also a key phosphorylation site of STAT3. Serine 727 is phosphorylated by multiple kinases including mitogen-activated kinases (MAPK), mammalian target of rapamycin kinase (mTOR), cyclin-dependent kinase 5 (CDK5) and nemo-like kinase (NLK) (Wakaharaet al.2012). Controversy exists around the effect of phosphorylation at serine 727 (Wakaharaet al.2012). There are reports that the transcriptional activity of STAT3 coincides with phosphorylation at serine 727. Some reports indicate that the transcriptional activity of STAT3 coincides with phosphorylation at serine 727 (Yokogamiet al.2000). Other reports show STAT3 activation independent of serine 727 (Wakahara et al. 2012). The role of serine 727 is likely dependent on the larger cellular context. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antiserum to STAT3 (pSer727) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
| Immunogen | Phospho specific-peptide corresponding to residues surrounding serine 727 of human STAT3 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Western Blotting | √ | 1/500 | 1/2000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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