Rabbit anti AKT2 (pSer474) antibody
货号:AHP2548
规格:50 µl
价格:3188
产品类型:免疫组化一抗
品牌:Bio-Rad
抗原:AKT2 (pSer474)
物种:其它
宿主:兔
抗体亚型:IgG
荧光染料:Purified
产品介绍 | | |
Rabbit anti AKT2 (pSer474) antibodyrecognizes AKT2 also known as RAC-beta serine/threonine-protein kinase and protein kinase B beta, when phosphorylated at serine 474. AKT2 is a serine/threonine protein kinase that plays a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. AKT2 is specifically involved in the insulin receptor signaling pathway. AKT2 is phosphorylated at serine 474 by (MAPK)-activated protein kinase-2, integrin-linked kinase, p38 MAPK, PKCα, PKCβII, mTORC2, double-stranded DNA-dependent PK, autophosphorylation and ATM. Phosphorylation at threonine 309 and serine 474 are required for full activation of AKT2 (Suizuet al.2009). |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to AKT2 (pSer474) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide50% Glycerol |
Immunogen | Phospho specific-peptide corresponding to residues surrounding serine 474 of human AKT2 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
Store at -20℃Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1/500 | 1/2000 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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