| 产品介绍 | | |
| Rabbit anti Human HUWE1 antibodyrecognizes human E3 ubiquitin-protein ligase HUWE1, also known as HUWE1, ARF-binding protein 1, ARF-BP1, HECT, UBA and WWE domain-containing protein 1, Homologous to E6AP carboxyl terminus homologous protein 9, HectH9, Large structure of UREB1, LASU1, Mcl-1 ubiquitin ligase E3, Mule or Upstream regulatory element-binding protein 1. HUWE1 is a 4374 amino acid DNA binding protein with a predicted molecular mass of ~480 kDa.HUWE1 is an E3 ubiquitin-protein ligase involved in mediating ubiquitination and subsequent proteasomal degradation of target proteins. HUWE1 is also involved in apoptosis through catalyzation of the poly-ubiquitination and degradation of MCL1, ubiquitination of the p53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Research indicates that HUWE1 regulates the abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation (Hallet al.2007). |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mouse | √ |
| Rat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human HUWE1 was raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Immunogen | A synthetic peptide corresponding to an 19 amino acid sequence from near the carboxy terminal of human HUWE1. |
| Approx. Protein Concentrations | Total protein concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
| Immunohistology - Paraffin1 | √ | 5.0ug/ml | |
| Western Blotting | √ | 2.0ug/ml | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.ImmunohistologyThis product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.Histology Positive Control TissueHuman brain (cortex) or human heart |
| 数据 |
| Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human HUWE1 (AHP2039). |
| 相关试剂 |
| Description | Product Code | Applications | Pack Size |
| Antigen Retrieval Buffer, pH8.0 | BUF025A | IHC(P) | 500 mL |
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB | 0.5 mL |
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