产品介绍 |
| Goat anti Human Noxa antibodyrecognizes human Noxa, a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis activation.Noxa is a transcriptional target of p53, p73 and BH3-only proteins, and competes with, and displaces, the proapoptotic proteins BAK1, and BIM, from the antiapoptotioc protein MCL1 (Bcl-2-related protein EAT/mcl1), thereby influencing apoptosis versus cell survival. |
产品详情 |
Target Species | Human |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to human NOXA was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Immunogen | Synthetic peptide sequence C-TQLRRFGDKLNFRQK from the internal region of Noxa (NP_066950.1). |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/16000 | |
Western Blotting | √ | 0.03 | 0.1ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP2170 detects a band of approximately 16kDa in human cerebellum cell lysates.Suitable loading control:Goat anti Human GAPDH; product code(AHP1064)
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可用二抗 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
文献 |
| 1.Yu, S.J.et al.(2013) MicroRNA-200a promotes anoikis resistance and metastasis by targeting YAP1 in human breast cancer.Clin Cancer Res. 19 (6): 1389-99.2.Ikezoe, T.et al.(2010) p53 is critical for the Aurora B kinase inhibitor-mediated apoptosis in acute myelogenous leukemia cells.Int J Hematol. 91: 69-77. |
QQ:1749072012