Sheep Anti 5-Methylcytosine (5-MeC)
货号:AHP1826Z
规格:0.1 mg
价格:2465
产品类型:免疫组化一抗
品牌:Bio-Rad
抗原:5-Methylcytosine (5-MeC)
物种:Broad
宿主:Sheep
抗体亚型:IgG
荧光染料:Purified
产品介绍 | | |
Sheep Anti 5-Methylcytosine (5-MeC)recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occuring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, wherease in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements (Heet al.2011).The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites (Morganet al.2004). Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers (Pfeiferet al.2005).Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry. |
产品详情 | | |
Target Species | Broad |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to 5-MeC was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | Keyhole limpet haemocyanin (KLH) conjugated 5-methylcytosine. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | | |
Immunohistology - Frozen1 | √ | 10ug/ml | |
Immunohistology - Paraffin2 | √ | 10ug/ml | |
Immunoprecipitation | √ | 25 | 100ug/ml |
Western Blotting | √ | 1:1000 | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail.2* Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C;E;F;WB | 1.5 ml |
Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F;IF | 0.1 mg |
Donkey anti Sheep/Goat IgG:HRP | STAR88P | C;E;P;WB | 1 ml |
文献 |
1. Hiura, H.et al.(2010) A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip.Nucleic Acids Res. 38 (15): 4929-45.2. Pontes, O.et al.(2009) RNA polymerase V functions inArabidopsisinterphase heterochromatin organization independently of the 24-nt siRNA-directed DNA methylation pathway.Mol Plant. 2: 700-10. |
技术参数 产品应用 IHC(F)*;IF;IP;IHC(P)*;WB
产品类型 Polyclonal Antibody
推荐用于 IHC(F)*;IF;IP;IHC(P)*;WB
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