| 产品介绍 | | |
| Rabbit anti Human CDKN2A antibodyrecognizes human cyclin-dependent kinase inhibitor 2A (CDKN2A), a 16 kDa protein belonging to the CDKN2 cyclin dependent kinase inhibitor family.CDKN2A acts as a negative regulator of cell proliferation by interacting strongly with CDK4 and CDK6, thus inhibiting their ability to interact with cyclin D and to phosphorylate the retinoblastoma protein.CDKN2A is an important tumour suppressor and defects in its production results in tumour formation in a wide range of tissues, including cutaneous malignant melanoma and Li-Fraumeni syndrome. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human CDKN2A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | A 19 amino acid peptide located near the amino terminus of human CDKN2A. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | |
Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1488 detects a band of approximately 20 kDa in mouse colon cell lysates.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Western blot analysis of mouse colon lysate probed with Rabbit anti Human CDKN2A (AHP1488) at 1(A) and 2(B) μg/ml. |
| 文献 |
| 1. Stone, S.et al.(1995) Complex structure and regulation of the P16 (MTS1) locus.Cancer Res. 55 (14): 2988-94.2. Kamb, A.et al.(1994) Analysis of the p16 gene (CDKN2) as a candidate for the chromosome 9p melanoma susceptibility locus.Nat Genet. 8 (1): 23-6.3. Kratzke, R.A.et al.(1995) Immunohistochemical analysis of the p16INK4 cyclin-dependent kinase inhibitor in malignant mesothelioma.J Natl Cancer Inst. 87 (24): 1870-5.4. Stott, F.J.et al.(1998) The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2.EMBO J. 17 (17): 5001-14. |
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