Goat anti Human GFAP antibody
货号：AHP1468
规格：0.1 mg
价格：5083
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：GFAP
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti Human GFAP antibodyrecognizes an epitope within the C-terminal (CT) region of human GFAP (glial fibrillary acidic protein), a class III intermediate filament (IF) protein specifically expressed by glial cells or cells of glial origin e.g astrocytes, ependymal cells and Schwann cells.GFAP plays a role in several cellular functions within the central nervous system (CNS), including cell structure and stability, communication, motility and mitosis, and is rapidly synthesized during astrogliosis, following trauma/injury. Mutations in the GFAP gene are responsible for the rare autosomal dominant disorder known asAlexander disease, resulting in the destruction of brain white matter and the formation of fibrous, eosinophilic deposits known as Rosenthal fibers. Characteristics of this disease are associated with transgenes and other mutation types in mouse. |
| 产品详情 | | |
Target Species | Purified IgG - liquid |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human GFAP (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide sequence C-DGEVIKESKQEHKD from the C-terminal region of GFAP (NP_002046.1). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/32000 |
Western Blotting | √ | 1.0 | 3.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1468 detects a band of approximately 48kDa in mouse brain cell lysates.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
Immunohistochemical staining of paraffin embedded human cerebellum following heat mediated antigen retrieval in citrate buffer pH 6.0 with Goat anti Human GFAP antibody at 2μgml-1followed by secondary anti Goat-HRP and DAB chromogen visualization |
| 文献 |
| 1. Gomes, F.C.et al.(1999) Glial fibrillary acidic protein (GFAP): modulation by growth factors and its implication in astrocyte differentiation.Braz J Med Biol Res. 32 (5): 619-31. |
技术参数 产品应用 ELISA;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;WB
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