Rabbit anti Rat Raf-1 (pSer642) antibody
货号：AHP1237
规格：0.1 ml
价格：5083
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Raf-1 (pSer642)
物种：其它
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Rat Raf-1 (pSer642) antibodyrecognizes Raf-1, also known as RAF proto-oncogene serine/threonine-protein kinase and proto-oncogene c-RAF (cRaf), when phosphorylated at serine 642. Raf-1is a ubiquitously expressed ~74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642. |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG - liquid |
| Antiserum Preparation | Antisera to rat Raf-1 (pSer 642) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared affinity chromatography. |
| Buffer Solution | 10mM Hepes pH7.5150mM NaCl |
| Immunogen | Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1. |
| Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | 1/1000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Gollob, J.A.et al.(2006) Role of Raf kinase in cancer: therapeutic potential of targeting the Raf/MEK/ERK signal transduction pathway.Semin Oncol. 33 (4): 392-406.2.Dougherty, M.K.et al.(2005) Regulation of Raf-1 by direct feedback phosphorylation.Mol Cell. 17 (2): 215-24. |
技术参数 产品应用 wb
产品类型 Polyclonal Antibody
推荐用于 wb
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