| 产品介绍 | | |
| Rabbit anti Human STEAP1 antibodyrecognizes STEAP1, also known as six transmembrane epithelial antigen of the prostate. STEAP1 is a ~39 kDa protein and member of a family of cell surface serpentine transmembrane antigens of the prostate.STEAP1 is predominantly expressed in human prostate and is up-regulated in several cancer lines including prostate, ovarian, cervical, breast, testicular, bladder, colon and Ewing sarcoma. It is significantly expressed at cell-cell junctions and has been shown to be a target of human CD8 T cells. This indicates that it could be used as a potential target for cancer immunotherapy. This antibody does not cross-react with other STEAP-proteins. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Antisera to human STEAP (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | An 18 amino acid peptide located near the c-terminal region of human STEAP1. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | | |
Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1438 detects a band of approximately 40kDa in human spleen cell lysates.
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| 数据 |
Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human STEAP1 (AHP1438) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0). |
| 文献 |
| 1. Hubert, R.S.et al.(1999) STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors.Proc Natl Acad Sci U S A. 96 (25): 14523-8.2. Alves, P.M.et al.(2006) STEAP, a prostate tumor antigen, is a target of human CD8+ T cells.Cancer Immunol Immunother. 55 (12): 1515-23. |
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