| 产品介绍 | | |
| Goat anti Human Ezrin antibodyrecognizes human ezrin, otherwise known as p81 or villin 2, Ezrin is a 586 amino acid ~80kDa cytoplasmic peripheral membrane protein containing a singleFERMdomain and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization (Nget al.2001). Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44 (Finnertyet al.2004). Ezrin is implicated in the metastatic capacity of various types of human tumors including breast, prostate, bone, endometrial and colon cancers (Ohtaniet al.1999,Elzaqheidet al.2008). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human ezrin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5mg/ml |
Immunogen | Peptide sequence C-QDYEEKTKKAER from the internal region of ezrin (NP_003370.2). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/16,000 |
Western Blotting | √ | 1.0 | 3.0ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1394 detects a band of approximately 80kDa in human placenta and duodenum cell lysates.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
| Western blotting analysis of Human Placenta lysate (35μg protein in RIPA buffer) probed using Goat anti Human ezrin antibody (AHP1394) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence. |
| 文献 |
| 1. Tsukita, S. & Yonemura, S. (1997) ERM (ezrin/radixin/moesin) family: from cytoskeleton to signal transduction.Curr Opin Cell Biol. 9 (1): 70-5. |
QQ:1749072012