| 产品介绍 | | |
| Rabbit anti human PlGF antibodyrecognizes human placental growth factor (PlGF). PlGF is a dimeric glycoprotein and member of the vascular endothelial growth factor (VEGF) family, involved in angiogenesis, and the stimulation of endothelial cell proliferation and migration.The three isoforms PlGF-1, PlGF-2 and PlGF-3 are present in most placental tissues and act through binding to the VEGFR-1/FLT1 receptor. The involvement of PlGF in angiogenesis has made PlGF a focus area in the study of inflammatory diseases such as rheumatoid arthritis and psoriasis, and PlGF is used as a biochemical marker in cardiovascular disease, and also detected at reduced levels during pre-eclampsia (Krausset al.2004). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to human PlGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
Immunogen | Recombinant human PLGF. |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
Immunohistology - Paraffin1 | √ | | 2.0ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- This purified human PLGF antibody may be used in Western Blotting applications under either reducing or non-reducing conditions.
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| 二抗相关产品 |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human PLGF (AHP1381) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0). |
| 文献 |
| 1. Maglione, D.et al.(1991) Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor.Proc Natl Acad Sci U S A. 88 (20): 9267-71. |
QQ:1749072012