Rabbit anti Human RFI antibody
货号：AHP1340
规格：0.1 mg
价格：2576
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原： RFI
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human RFI antibodydetects human Ring Finger homologous to Inhibitor of apoptosis protein (hRFI), an anti-apoptotic protein which has been shown to be a novel modulator of NF-kB activation, leading to the upregulation of Bcl2 and BclXL. It is also thought to possess E3 ubiquitin protein ligase activity and has been shown to target caspases 8 and 10 for proteasomal degradation.hRFI is expressed ubiquitously, and has been localized to both the nucleus and the cytoplasm. hRFI is also expressed in oesophageal, gastric and colorectal cancers, suggesting a possible association between hRFI and the development of digestive tract cancers. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human RFI were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | Recombinant protein corresponding to amino acids 1-373 of human RFI. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1800 | 1/4000 |
Western Blotting | √ | 1/100 | 1/600 |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1340 detects a band of approximately 42kDa.
|
| 二抗相关产品 |
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Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 文献 |
| 1. Konishi, T.et al.(2006) Overexpression of hRFI inhibits 5-fluorouracil-induced apoptosis in colorectal cancer cells via activation of NF-kappaB and upregulation of BCL-2 and BCL-XL.Oncogene. 25 (22): 3160-9. |
技术参数 产品应用 ELISA;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;WB
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