| 产品介绍 | | |
| Rabbit anti Human Cspase-14 antibodyrecognizes the C-terminal (CT) region of human caspase-14; a ~27 kDa cysteine protease which is activated by zymogen processing during cellular differentiation. It is highly expressed by keratinocytes and trophoblast cells of the placenta, with lower expression in the choroid plexus, the pigmented layer of the retina, and hair follicles.Expression of the caspase-14 gene has recently been identified in epithelial tumours, including cervical and bladder carcinoma. The biological function of caspase-14 in tumour and normal tissue remains to be classified. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to Caspase-14 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1 mg/ml |
Immunogen | A 16 amino acid peptide from near the carboxy-terminus of human Caspase-14. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1 | 2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1175 detects a band of approximately 27kDa in Jurkat cell lysates.
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| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Western blot analysis of whole cell lysate from Jurkat cells probed with Rabbit anti caspase-14 (AHP1175) either in the absence (A) or presence (B) of blocking peptide. |
| 文献 |
| 1. Park, K.et al.(2006) Expression and characterization of constitutively active human caspase-14.Biochem Biophys Res Commun. 347 (4): 941-8.2. Koenig, U.et al.(2005) Aberrant expression of caspase-14 in epithelial tumors.Biochem Biophys Res Commun. 335 (2): 309-13.3. White, L.et al.(2007) Caspase-14: a new player in cytotrophoblast differentiation.Reprod Biomed Online. 14 (3): 300-7. |
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