| 产品介绍 | | |
| Rabbit anti Human SphK1 antibodydetects epitopes within the N-terminal region human SphK1, also known sphingosine kinase 1. SphK1 catalyzes the phosphorylation of sphingosine 1-phosphate; an important lipid mediator with multiple functions. In light of this, SphK1 has been implicated in regulation of Ca2+homoeostasis, inflammation, immunity and cancer growth. However, its central function is thought to be in the development of the cardiovascular and central nervous system. SphK1 has a molecular weight of ~42 kDa. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to SphK1 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | Synthetic peptide corresponding to the N-terminal domain of human SphK1, conjugated to KLH. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunohistology - Paraffin1 | √ | 5 | 15ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Adrenal, Pheochromocytes.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Staining of formalin fixed, paraffin embedded human adrenal medullary pheochromocytes with Rabbit anti Human SPHK1 (AHP1162) |
| 文献 |
| 1. Maceyka, M.et al.(2005) SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism.J Biol Chem. 280 (44): 37118-29.2. Alemany, R.et al.(2007) Regulation and functional roles of sphingosine kinases.Naunyn Schmiedebergs Arch Pharmacol. 374 (5-6): 413-28. |
QQ:1749072012