| 产品介绍 | | |
| Goat anti Human RXR alpha antibodydetects an epitope within the internal region of retinoid X receptor alpha (RXR alpha). RXR alpha is a ~54 kDa member of the steroid/ thyroid receptor superfamily of transcriptional regulators, and is a nuclear receptor that mediates the biological effects of retinoids through its involvement in retinoic acid-mediated gene activation. RXR alpha regulates gene transcription by recruiting coactivators or corepressors to their promoter sequences.RXRs play important roles in multiple signalling pathways, including cell growth, differentiation, homeostasis, metabolism, and development.Goat anti Human RXR alpha antibody does not cross-react with either RXR beta or gamma. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antisera to human RXR alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | 0.02%Sodium Azide 0.5%Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Immunogen | Peptide sequence CQVNSSLTSPTGRGSM corresponding to the internal region of RXR alpha (NP_002948). |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/15000 | 1/20000 |
Western Blotting | √ | 0.5 | 3ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1116 detects a band of approximately 55kDa in human epidermoid carcinoma A431 lysates. A minimum incubation time of 1 hour is recommended with this antibody.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
| Western blot analysis of HeLa cell, nuclear lysate (A) + pre-incubation with immunizing peptide (B). K562 cell, nuclear lysate (C) + immunizing peptide (D). 35μg protein in RIPA buffer for each lane. Detection is by chemiluminescence. |
| 文献 |
| References for RXR Alpha antibody1. Pestka, A.et al.(2011) Retinoid X receptor α and retinoids are key regulators in apoptosis of trophoblasts of patients with recurrent miscarriages.J Mol Endocrinol. 47 (2): 145-56.2. Zhang, Z.et al.(2010) Constitutive Activation of the Mitogen Activated Protein Kinase Pathway Impairs Vitamin D Signaling in Human Prostate Epithelial CellsJ Cell Physiol. 224: 433-42.Further Reading1. Lee, H.Y.et al.(2000) Stress pathway activation induces phosphorylation of retinoid X receptor.J Biol Chem. 275 (41): 32193-9.2. Ghosh, J.C.et al.(2002) Interactions that determine the assembly of a retinoid X receptor/corepressor complex.Proc Natl Acad Sci U S A. 99 (9): 5842-7.3. Konta, T.et al.(2001) Selective roles of retinoic acid receptor and retinoid x receptor in the suppression of apoptosis by all-trans-retinoic acid.J Biol Chem. 276 (16): 12697-701. |
QQ:1749072012