Rabbit anti DYKDDDDK Tag:HRP
货号：AHP1074P
规格：0.1 mg
价格：6885
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：DYKDDDDK Tag
物种：Synthetic Peptide
宿主：兔
抗体亚型：IgG
荧光染料：HRP
| 产品介绍 | | |
| Rabbit anti DYKDDDDK Tag antibodyrecognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins. |
| 产品详情 | | |
Target Species | Synthetic Peptide |
Product Form | Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized |
Antiserum Preparation | Antisera to peptide DYKDDDDK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.01% Gentamicin Sulfate1% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 1mg/ml |
Immunogen | Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml deionised water. For long term storage at -20oC, add glycerol to a final concentration of 50%. |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/10,000 | 1/50,000 |
Western Blotting | √ | 1/1,000 | 1/5,000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 相关产品 |
Description | Product Code | Applications | Pack Size |
| AbGUARD® HRP Stabilizer Plus | BUF052A | C;E;P;WB | 100 ml |
| AbGUARD® HRP Stabilizer Plus | BUF052B | C;E;P;WB | 500 ml |
| AbGUARD® HRP Stabilizer Plus | BUF052C | C;E;P;WB | 1000 ml |
| TMB Signal+ | BUF054A | ELISA | 100 ml |
| TMB Core | BUF056A | ELISA | 100 ml |
| TMB Core+ | BUF062A | ELISA | 100 ml |
| 文献 |
| References for DYKDDDDK Tag antibody1. Keller, B.et al.(2016) Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT.J Exp Med. May 30. pii: jem.20151110. [Epub ahead of print]Further Reading1. Hopp, T.P.et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification.Bio/Technology 6: 1204-10. |
技术参数 产品应用 ELISA;WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;WB
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