| 产品介绍 | | |
| Rabbit anti Rat GRO beta/MIP-2 antibodyrecognizes rat GRO beta/MIP-2, also known CXCL2, an 8kD chemokine which promotes neutrophil chemotaxis and degranulation and is expressed by monocytes, fibroblasts, endothelial cells, synovial cell and several tumour cell lines. |
| 产品详情 | | |
Target Species | Rat |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to rat GRO beta/MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | Recombinant rat GRO beta/MIP-2. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Reconstitution | Reconstitute with 0.1 ml distilled water. The addition of 0.09% sodium azide is recommended for long term storage.N.B.For functional studies do not add sodium azide. |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 0.5ug/ml |
Functional Assays | √ | 3.0ug/ml | 5.0ug/ml |
Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1. Vallès, A.et al.(2006) Differential regulation of the CXCR2 chemokine network in rat brain trauma: implications forneuroimmune interactions and neuronal survival.Neurobiol Dis.22: 312-22. |
QQ:1749072012