| 产品介绍 | | |
| Rabbit anti Rat CXCL1 antibodyrecognizes rat CXCL1 also known as GRO1 and CINC-1, an ~8 kDa cytokine which promotes neutrophil chemotaxis and degranulation and is expressed predominantly by monocytes after activation, but is also expressed by fibroblasts, endothelial cells, synovial cells and several tumour cell lines. |
| 产品详情 | | |
Target Species | Rat |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antiserum to CXCL1 was raised by repeated immunisation of rabbits with highly purified antigen. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution. |
Immunogen | Recombinant rat CXCL1. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| Preparation | Purified IgG prepared by affinity chromatography |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5ug/ml | 2.0ug/ml |
Functional Assays | √ | 5ug/ml | 10ug/ml |
Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls. |
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| 文献 |
| 1. Vallès, A.et al.(2006) Differential regulation of the CXCR2 chemokine network in rat brain trauma: implications forneuroimmune interactions and neuronal survival.Neurobiol Dis.22: 312-22. |
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