Goat anti Mouse CD197 antibody
货号：AAM70
规格：50 µg
价格：3383
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD197
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Goat anti Mouse CD197 antibodyrecognizes mouse C-C chemokine receptor type 7, also known as CD197, CCR7, Epstein-Barr virus-induced G-protein coupled receptor 1 or MIP-3 beta receptor. CD197 is a 378 amino acid ~43 kDa multi pass type III transmembrane glycoprotein and member of the G-protein coupled receptor 1 family (UniProt: P74447). CD197 is expressed by various lymphoid tissues, activated B and T cells, naive T cells, NK cells and dendritic cells (DCs), which acts a high-affinity receptor for the chemokines MIP-3beta (CCL19) and CCL21.Ligand binding of CD197 plays a vital role in the homing of naive T cells and antigen-loaded DCs into secondary organs. Expression of CD197 is strongly up-regulated in B cells infected with Epstein-Barr virus (EBV) and T cells infected with herpesvirus 6 or 7. Goat anti Mouse CD197 antibody can be used to distinguish between central memory and effector memory T cells and is suitable for use in immunocytochemistry. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to mouse CD197 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1% Sodium Azide, 0.1% Bovine Serum Albumin. |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | Synthetic peptide DPGKPRKNVLVVALLVIFQVC corresponding to amino acids 2-22 of mouse CD197. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/100000 | |
Flow Cytometry | √ | 1/10 | |
| Functional Assays | √ | 8 ug | |
| Immunohistology - Paraffin | √ | 1/250 | |
Western Blotting | √ | 1/1000 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.ELISAThis product is suitable for use in indirect ELISA applications.Histology Positive Control TissueSpleen. |
| 二抗产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | Flow | 1 mg |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C;E;WB | 1 mg |
| 数据 |
| Paraffin embedded mouse spleen stained with Goat anti mouse CD197 (AAM70). |
技术参数 产品应用 ELISA;Flow;FN;IHC(P);WB
产品类型 Polyclonal Antibody
推荐用于 ELISA;Flow;FN;IHC(P);WB
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