| 产品介绍 | | |
| Rabbit anti Mouse RANTES antibodyrecognizes mouse RANTES, also known as CCL5.RANTES is expressed by circulating T cells and T cells in culture. RANTES is chemotactic for peripheral blood monocytes. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to mouse RANTES were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution |
Immunogen | Recombinant mouse RANTES. |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
Reconstitution | Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 0.5ug/ml |
Functional Assays | √ | 6.0ug/ml | 8.0ug/ml |
| Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1. Ruddell, R.G.et al.(2009) Lymphotoxin-beta receptor signaling regulates hepatic stellate cell function and wound healing in a murine model of chronic liver injury.Hepatology. 49: 227-39.2. Keepers, T.R.et al.(2007) Monocyte chemoattractant protein 1, macrophage inflammatory protein 1 alpha, and RANTES recruit macrophages to the kidney in a mouse model of hemolytic-uremic syndrome.Infect Immun. 75: 1229-36.3. Hildebrandt, G.C.et al.(2005) Donor T-cell production of RANTES significantly contributes to the development of idiopathic pneumonia syndrome after allogeneic stem cell transplantation.Blood. 105: 2249-57. |
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