| 产品介绍 | | |
| Rabbit anti Mouse MIP-2 antibodyrecognizes mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2.MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 0.1 mg/ml after reconstitution. |
Immunogen | Recombinant mouse MIP-2 |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 0.5ug/ml |
Functional Assays | √ | 0.50ug/ml | 0.90ug/ml |
| Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin1 | √ | | |
Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.ELISARabbit anti Mouse MIP-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with biotinylated Rabbit anti mouse MIP-2 antibody (AAM48B) as the detection antibody and recombinant Mouse MIP-2 protein (PMP55) as a standard.Western BlottingRabbit anti Mouse MIP-2 antibody may be used under either reducing or non-reducing conditions with recombinant Mouse MIP-2 (PMP55) protein as a positive control. |
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| 文献 |
| 1. Tittel, A.P.et al.(2011) Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.J Am Soc Nephrol. 22 (8): 1435-41.2. Roche, J.K.et al.(2007) CXCL1/KC and CXCL2/MIP-2 are critical effectors and potential targets for therapy of Escherichia coli O157:H7-associated renal inflammation.Am J Pathol. 170: 526-37.3. Di Carlo, E.et al.(2000) The combined action of IL-15 and IL-12 gene transfer can induce tumor cell rejection without T and NK cell involvement.J Immunol. 165: 3111-8.4. Cavallo, F.et al(2001) Interleukin 12-activated lymphocytes influence tumor genetic programs.Cancer Res. 61: 3518-23.5. González-López, A.et al.(2011) Inflammation and matrix remodeling during repair of ventilator-induced lung injury.Am J Physiol Lung Cell Mol Physiol. 301: L500-9.6. Lazic, M.et al.(2014) Differential regulation of inflammation and apoptosis in Fas-resistant hepatocyte-specific Bid-deficient mice.J Hepatol. 61: 107-15.7. Nicholas, J.et al.(2015) Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice.Innate Immun. 21 (3): 266-74.8. Yoshida, T.et al.(2014) Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.Genes Cells. 19: 842-52. |
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