| 产品介绍 | | |
| Rabbit anti Mouse CXCL1 antibodyrecognizes mouse CXCL1 also known as GRO1 and KC, a ~7.8 kDa chemokine involved in neutrophil chemotaxis and activation, and expressed in a range of cells including monocytes, macrophages and vascular endothelial cells. |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution. |
Immunogen | Recombinant mouse CXCL1 |
Regulatory | For research purposes only |
Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
Western Blotting | √ | 0.1 | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAAAM40 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA withAAM40Bas the detection antibody andPMP66as the standard.Western BlottingAAM40 may be used under either reducing or non-reducing western blotting conditions withPMP66as the positive control. |
| 二抗相关产品 |
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| 文献 |
| 1. Liu, H.et al.(2017) NLRP3 inflammasome induced liver graft injury through activation of telomere-independent RAP1/KC axis.J Pathol. 242 (3): 284-96. |
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