| 产品介绍 | | |
| Rabbit anti Mouse Interleukin-4 antibodyrecognizes mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages (Crawfordet al.1987).Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching (Winteret al.1987). IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajimaet al.1991,Hudaket al.1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor (Steinet al.1992). |
| 产品详情 | | |
Target Species | Mouse |
Product Form | Purified IgG - lyophilized |
Antiserum Preparation | Antisera to mouse IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml after reconstitution. |
Immunogen | Recombinant mouse IL-4 |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
Reconstitution | Reconstitute with 0.1ml distilled water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.For long term storage the addition of 0.09% sodium azide is recommended..NB: For functional studies do not add azide. |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 0.5ug/ml |
Functional Assays | √ | 0.25ug/ml | 0.40ug/ml |
Western Blotting | √ | 0.1ug/ml | 0.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.ELISAAAM36 may be used in an indirect ELISA withPMP81as the standard. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
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| 文献 |
| 1. de Barros, V.E.D.et al.(2011) An experimental model of meningoencephalomyelitis by Rocio flavivirus in BALB/c mice: inflammatory response, cytokine production, and histopathology.Am J Trop Med Hyg. 85 (2): 363-73.2. Kim, S.R.et al.(2013) Endoplasmic reticulum stress influences bronchial asthma pathogenesis by modulating nuclear factor κB activation.J Allergy Clin Immunol. pii: S0091-6749(13)01382-1. |
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